1·Methods Cultured monolayer-confluent human RPE cells were scraped with a trephine and a cotton stick, and set up the injured model of RPE cells with round scraped area.
方法利用体外培养的单层近融合期人RPE细胞,采用棉签和角膜移植用环钻做圆形细胞刮伤区,建立体外RPE细胞损伤模型。
2·Traditional scheme is core decompression technique using trephine.
传统的治疗方法是以环钻法为主的髓心减压技术。