1·Objective: To set the measuring method of tyrosine hydroxylase activity in the brain of conscious rats.
中文摘要:目的:建立清醒自由活动大鼠脑内酪氨酸羟化酶活性的测定方法。
2·Methods: By using GFP gene as a marker, to observe expression of human tyrosine hydroxylase type I (HTH1) gene in vitro or in brains.
方法:以绿色荧光蛋白(GFP)作为标记,观察hth_1基因在体外和脑内的表达。
3·AIM: To observe the relationship between the drug dependence behavior and levels of tyrosine hydroxylase (th) in drug acquisition process.
目的:探讨药物依赖行为和酪氨酸羟化酶在成瘾过程中的相互关系。
4·Methods To introduce DNA encoding the wild- type and mutant channel into immortalized tyrosine hydroxylase- positive CNS- derived neurons, CAD cells.
方法给一类酪氨酸脱氢酶阳性的神经细胞(一种中枢神经源细胞),又称CAD细胞,引入由DNA编码的野生型和突变型离子通道。
5·Objective:To observe the expression of Tyrosine Hydroxylase(TH) of Hypothalamus in post-stroke depression rat model and effects of Yishen Tiaoqi decoction.
目的:观察脑缺血后抑郁模型大鼠下丘脑酪胺酸羟化酶(TH)表达及益肾调气方药的干预作用。
6·CONCLUSION: NURR1 gene combined with NSC can effectively ameliorate PD models symptoms and elevate the number of tyrosine hydroxylase positive neurons after transplantation.
结论:NURR1基因结合神经干细胞有效改善了帕金森病模型症状,提高移植后酪氨酸羟化酶阳性神经元细胞的数量。
7·Methods: Immunohistochemistry technology and high-pressure liquid chromatography with electrochemical detector (HPLC-EC) were used to evaluated tyrosine hydroxylase (TH) and DA .
方法:采用免疫组化技术和高效液相- 电化学法( HPLC - EC)分别对酪氨酸羟化酶(TH)、DA进行了含量测定。
8·Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation (2, 3).
增加EGR1表达可以激活其他信号分子的转录,包括CDK5和酪氨酸羟化酶,对神经细胞生长和分化有长期影响(2,3)。