1·Methods Dental pulp stem cells were cultured in vitro, purified and then subcultured .
密度梯度离心法体外培养骨髓间充质干细胞。
2·Methods: Fibroblasts from human skin and hypertrophic scar were primary cultured and subcultured.
方法：人皮肤及瘢痕成纤维细胞原代、代培养。
3·There were some differences in water soluble proteins for the natural, primary cultured and subcultured potatoes.
自然生长、初代培养和继代培养的马铃薯再生体的水溶性蛋白之间存在着一些差异。
4·Somatic embryos were developed to normal seedlings when subcultured on MS medium without plant growth regulators.
体细胞胚胎可在不含植物生长调节剂的MS固体培养基上形成正常植株。
5·After the cells were subcultured, the frozen storage didn't influence their growth, morphology, and genetic characteristics.
通过冻存后复苏的细胞继续培养后发现，冻存对其生长、形态、遗传学等特征无明显影响。
6·Methods With an operating microscope and microsurgical instruments, rat's mesenteric lymphatics were isolated and the endothelium were primarily cultured and subcultured.
方法利用手术显微镜及显微手术器械分离大鼠肠系膜淋巴管。 采用植块培养法进行内皮细胞原代培养并传代培养。
7·The special growth rate of Taxus cell subcultured on logarithmic phase was 1.6 folds of that on stationary phase and the production of taxol was increased by nearly 4 times;
在细胞生长的对数期继代较静止期继代的细胞增长率提高1.6倍，同时紫杉醇含量提高了近4倍；
8·Furthermore, these hESC-derived keratinocytes could be subcultured to obtain relatively pure keratinocyte populations that retained the capacity to terminally differentiate.
此外，这些人的胚胎干细胞来源的角质细胞可以再次培养以获得纯的角质细胞，这些角质细胞能够保持终极分化能力。
9·The multiple bud clumps subcultured were a series of salt filtration and offspring breeding selection. Then salt-tolerant plantlets were regenerated and T_2 lines were obtained.
本实验室以继代培养的丛生芽块为材料，经过连续加盐筛选、后代选育，获得了一批耐盐株系。
10·Rat triceps brachii muscle was acquired to separate skeletal muscle satellite cells with the two-step method of collegenase-1 and trypsin and were cultured and subcultured in vitro.
取大鼠肱三头肌，采用二步消化法分离骨骼肌卫星细胞，体外进行原代培养和传代培养。