1·On the 8th day mice except in normal group were anesthetized and ligated bilateral common carotid artery and pneumogastric nerve so as to establish models of acute ischemia.
第8天，除正常组外其余小鼠麻醉后，结扎双侧颈总动脉和迷走神经，造成小鼠急性脑缺血模型。