1·Methods: Hemolyzed rabbit red blood cell was used as the sample.
方法：兔红细胞溶血后为样品。
2·Methods:Red blood cell(RBC) was isolated from the anti-coagulated fresh pig blood, It was hemolyzed by heated water, then heme was extracted with 3% acetone-hydrochloric acid.
方法：采用新鲜猪血抗凝离心后取血球，水浴加热溶血后，用3%盐酸丙酮提取血红素，提取液加鞣酸/氯化锶沉淀分离出血红素。