1·Methods Colorate the slice of the rat dental pulp by the immunohistochemical methods. Observe the distribution of SP-IR and CGRP-IR nerve fibers by microscope.
方法:利用免疫组织化学方法对大鼠牙髓组织切片进行染色,在显微镜下观察SP和CGRP免疫阳性神经纤维的分布。
2·Method: Colorate the slice of the rat dental hard tissues and peridental tissue by the method of hybridization in situ. Observe the location of CGRP1 receptor by microscope.
方法:利用原位杂交方法对大鼠牙齿及牙周组织切片进行染色,在显微镜下观察CGRP1受体的定位。