1·方法：用一条锚定的IS 1541内部锚定引物和一条随机引物进行PCR扩增；
Methods: PCR with an anchoring primer in IS 1541 and random primer;
2·徜徉漫步于最古老的阿姆斯特丹（荷兰首都）运河，周围建筑物大概都是1541和1603年建造的，这让人不禁想象早在17世纪这个城市繁华的样子。
Strolling beside Amsterdam’s oldest canals, where buildings carry dates like 1541 and 1603, it is easy to imagine the city’s prosperity in the 17th century.
3·闲庭信步在阿姆斯特丹最古老的运河边，两岸是建筑在1541年至1603年之间的建筑，这不难想象在17世纪这个城市是一个怎样的一派繁华。
STROLLING beside Amsterdam's oldest canals, where buildings carry dates like 1541 and 1603, it is easy to imagine the city's prosperity in the 17th century.
4·布拉迪斯拉瓦：捷克斯洛伐克南部城市，位于奥地利和匈牙利边界附近多瑙河畔。1541至1784年间曾是匈牙利首都。人口409，100。
A city of southern Czechoslovakia on the Danube River near the Austrian and Hungarian borders. It was the capital of Hungary from 1541 to 1784. Population, 409,100.